Genomics Protocols

Genomic DNA preparation for Illumina Sequencing
pdf

Nextera library preparation
Our modified version of the standard Nextera protocol. Adapted from Baym et al. PLoS ONE 2015.
pdf

Constructing bulk-segregant pools.
pdf

Harvesting cells for RNA
Adapted from Maitreya Dunham.
pdf

RNA preparation
Adapted from Maitreya Dunham.
pdf

RNA labeling
Adapted from Maitreya Dunham.
pdf

Assays

Fluctuation assay
pdf

Determining ploidy by DNA content staining

High-thoughput killer assay

High-thoughput flow cytometry-based fitness assay

Basic Protocols

Yeast transformation
pdf

Quick yeast genomic DNA preparation
pdf

PCR
pdf

Media recipes
pdf